Haploid mammalian cell lines are useful for forward genetics experiments, the idea being that new phenotypes (ie induced by mutations or knock-out) would be exposed immediately.
I haven’t been able to find good lists of such cell lines, so instead I have compiled a non-exhaustive list here. Very few are available, even though haploid cancers are known to arise in humans and some authors mention that many cell lines become hypodiploid or even near-haploid as they are passaged. If anybody knows of more, I would be happy to know.
| Name | Cancer | Non-haploid chromosomes | Reference |
|---|---|---|---|
| KBM-7 | Chronic myeloid leukemia | 8, 15*, Y* | Kotecki et al, 1999 |
| HAP1 | Derived from KBM-7, but fibroblast-like | Carette et al, 2011 | |
| NALM-16 | Acute lymphoblastic leukemia | ? | Kohno et al, 1980 |
| MMLAL | Myeloma | 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 18, 19, 20, 21, 22, X | Wong et al, 2013 |
* Varying descriptions as to whether these chromosomes are diploid or not.
References
1. Haploid vs diplod image from Wikimedia user Ehamberg.
2. Haploid genomes illustrate epigenetic constraints and gene dosage effects in mammals, Epigenetics & Chromatin 2013, Leeb and Wutz.
Skriv et svar